Published Jan 18, 2018


Positioning problems of scanning electrochemical microscopy (SECM) are very important by means of living cells damaging by moving ultramicroelectrode (UME). The working principle and operating modes of SECM are introduced. Investigation of redox activity of living cells are outlined. Problems, which arise in investigations of living cells by constant distance and constant height modes are discussed. Technical challenges and advances in application of SECM in living cell investigations are provided.


Tinkamas skenuojančiojo elektrocheminio mikroskopo (SECM) ultramikroelektrodo (UME) pozicionavimas yra aktualus eksperimentuojant su gyvomis ląstelėmis, nes gali pažeisti ląstelių paviršių. Šiame straipsnyje pateikiami SECM veikimo principas ir darbo režimai. Nagrinėjamas gyvų ląstelių oksidavimo ir redukavimo aktyvumas. Straipnyje pateikiama problemų, kurių kyla matuojant pastovaus aukščio ir pastovaus atstumo metodais, analizė. Pateikiami techniniai pozicionavimo sprendimai, iššūkiai ir progresas, taikant matuoti SECM gyvoms ląstelėms.

Reikšminiai žodžiai: skenuojantysis elektrocheminis mikroskopas, gyvos ląstelės, ultramikroelektrodas, žmogaus ląstelės, priartėjimo kreivės, šlyties jėgos SECM.



scanning electrochemical microscopy, living cells, ultramicroelectrode, human cells, approaching curves, shear- force SECM

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