Development of planar electrodes for real-time electroporation / Planariųjų elektrodų modelis realiojo laiko elektroporacijos tyrimams


In this paper the concept of planar electrodes for real-time electroporation on a microscope stage is presented and the structure is analyzed using finite element method (FEM) analysis. A multiparametric investigation of the chip topology is performed in COMSOL Multiphysics environment to define the configuration of electrodes, electric field distribution and other electroporation parameters to ensure a homogeneous cell exposure. Based on the simulation results, an optimal electrode configuration, which is suitable for the investigation of the permeabilization thresholds during electroporation, is proposed.


Šiame straipsnyje pristatomas planariųjų elektrodų, skirtų realiojo laiko elektroporacijos tyrimams, ant mikroskopo objektinio staliuko baigtinių elementų modelis. Pasiūlytas daugiaparametrio elektrodų lusto topologijos tyrimas atliekamas „COMSOL Multiphysics“ aplinkoje, siekiant nustatyti tinkamą elektrodų konfigūraciją, elektrinio lauko pasiskirstymą ir kitus elektroporacijos parametrus, kuriems esant būtų užtikrintas tolygus ląstelių poveikis. Remiantis modeliavimo rezultatais, siūloma optimali elektrodų konfigūracija, kuri užtikrina pakankamą permeabilizacijos slenkstinę įtampą elektroporacijos tyrimų metu.

Reikšminiai žodžiai: elektroporacija, permeabilizacija, elektrinis laukas, planarieji elektrodai, COMSOL modelis, modeliavimas.

Keyword : electroporation, electropermeabilization, electric field, planar electrodes, COMSOL model, simulation

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Oct 9, 2018
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